If you want some proof to show pro-COVID shot nay-sayers that these shots are detrimental to normal functioning immune responses, read them this. Before I get into this paper that should stop the presses and provide a one-way ticket for a lot of people to prison for the rest of their lives (as a best punishment), I refer to an earlier article that I wrote on immunological tolerance. I wrote this piece on July 10th, 2022, and now this group, who I may add have NO CONFLICTS OF INTEREST, have confirmed a mechanism of action of spike tolerance and so yes, the spike protein might be capable of destroying tolerance itself.
A paper was published in Science Immunology on December 22, 2022 entitled: “Class switch towards non-inflammatory, spike-specific IgG4 antibodies after repeated SARS-CoV-2 mRNA vaccination”1 and it explains in wonderful detail how a class of antibody that commands a non-inflammatory response (more like tolerizing) is prominent in people who have been repeatedly injected with the modified mRNA COVID-19 injectable products.
Translation: Instead of the intended pool of spike-specific neutralizing IgG antibodies being dominant in multiply-injected people, a pool of antibodies associated with spike-specific tolerance are dominant in multiply-injected people.
Besides the tolerizing capacity, they also showed that the phagocytic enabling capacities were much reduced overall. These activities lead to clearance of viral pathogens. Reduce them → reduction in viral clearance capacity.
Importantly, this class switch was associated with a reduced capacity of the spike-specific antibodies to mediate antibody-dependent cellular phagocytosis and complement deposition.
They did not see this result in the case of the non-mRNA injectable product ChAdOx1 (adenoviral vector-based vaccine) or for tetanus toxoid and Respiratory Syncytial Virus (RSV). They checked. This confirms that this class switch to this tolerizing antibody subclass is specific to the effects of repeated injections with the Comirnaty products (we’ll have to check the Moderna mRNA-1273 products) and more importantly, that is not a typical consequence of repeat antigen exposure from either natural infections and vaccination.
To be clear, this wasn’t a ‘maybe the antibody profile was a little different’ type situation. This was a ‘whoa there’s a 480% increase in spike-specific antibodies between the 2nd and 3rd injections’ situation.
IgG4 antibodies among all spike-specific IgG antibodies rose on average from 0.04% shortly after the second vaccination to 19.27% late after the third vaccination.
Before I get into some of the details of what they did in this paper, some background is required.
Background on IgG1 and IgG4
Ig stands for immunoglobulin. These proteins are also called antibodies and there are 5 major types called IgA (the first line mucous guns: tears, saliva, mucous, blood-related), IgG (the plentiful guns: all body fluids related – especially blood/plasma), IgM (the big guns: blood and lymph-related), IgE (the allergic guns: lungs, skin, and mucous membrane-related) and IgD (the who-knows guns: gut-related?). The IgG antibody type has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. I would like to focus to the first and last subclasses.
IgG1 is a subclass of antibody that responds to the presence of soluble proteins antigens and membrane proteins, so these are the guys normally associated with high levels in serum during and after viral infections. Deficiencies in this subclass can be associated with recurrent infections.2
IgG4 is a subclass of antibody that responds to repeated or long-term exposure to antigens, and ‘may become the dominant subclass’ as a result of this repeated exposure. Class switching to IgG4 may be modulated by Interleukin-10 (IL-10) and thus links this subclass of antibody to tolerance induction.3 It is really interesting that IgG4 may also represent the dominant antibody subclass in immune responses to the essential blood clotting proteins factor VIII and IX. Parasitic infections have also been found to be associated with the formation of IgG4 antibodies. (See reference #2).
*Regulation of IgG4 production is dependent on help by T-helper type 2 (Th2) cells and thus the IgG4 response is largely restricted to non-microbial antigens, like self-made spike proteins.4
Let’s get into some of the details of what they did. First, it is important to know that they looked at 2 independent test groups (N = 29 and N = 38) of healthcare workers who were injected 3 times with the Comirnaty product – the people who likely are exposed the most to all sorts of pathogens and thus, require the most robust defenses. They compared these two cohorts to another cohort of people who were injected with the adenoviral vector-based vaccine ChAdOx1 (AZD1222, Vaxzevria) and then got one shot of the Comirnaty product, and thus were able to distinguish between the specific antibody responses made between these cohorts and thus properly implicate the mRNA shots. Well, at least the Comirnaty shit. I mean shot.
In the following screenshot of Figure 1 B in the paper, they show data for 29 people who all had 3 doses of mRNA product. They measured the different IgG subclasses using flow cytometry and ‘recombinant monoclonal receptor binding domain (RBD)-antibodies as a standard’. For ease of eyes, they show the statistical comparison between the post 2nd data (10 days after 2nd dose), the follow-up (FU) post second (that included measurements after 210 days) and post 3rd (10 days after 3rd dose). The results speak for themselves.
Figure 1: Longitudinal analyses of vaccine induced antibody response. Figure 1 from paper https://www.science.org/doi/10.1126/sciimmunol.ade2798
There are 4 plots in Figure 1 showing the antibody responses and measurements for IgG1, IgG2, IgG3 and IgG4 following injections with the Comirnaty products. I want to draw attention to the bottom right plot. This plot clearly shows the difference in the IgG4 antibody response in these people following the 3rd shot. Although IgG1 also attains high levels post 3rd dose (IgG1 is important for clearing viral pathogens), it concerning that the IgG4 level is so high due to its function as a tolerizing antibody, as opposed to a ‘clearance’ antibody.
In four individuals IgG4 even became the most prominent IgG subclass after the third immunization. Specifically in individuals having experienced an additional infection, IgG4 antibodies accounted for 40–80% of all anti-S antibodies.
Here’s a plot from Figure S1 in the Supplementary Figures that sums it up pretty good. This one also indicates the changing antibody subclass profile for people who experienced breakthrough infections.
Figure 2: Figure S1: Longitudinal analyses of vaccine-induced antibody responses showing the relative abundance of the different anti-S IgG subclasses is shown for each individual for the indicated time points. Individuals who have experienced a breakthrough infection between the two
time points post 3rd and FU 3rd are indicated by grey dots. From Supplementary Materials of https://www.science.org/doi/10.1126/sciimmunol.ade2798
They also characterized the spike-specific memory B cells according to their IgG subclasses using flow cytometry and the frequencies of IgG4 spike-specific memory B cells using scRNA-seq to check mirroring of serological IgG4 anti-spike levels. All confirmatory.